Journal: Biotechnology and Applied Biochemistry

Article Title: Construction and optimization of an efficient amplification method of a random ssDNA library by asymmetric emulsion PCR

doi: 10.1002/bab.1467

Figure Lengend Snippet: Schematic diagram of asymmetric emulsion PCR. The different types of ssDNA, which marked with different colors, cannot touch each other in the process of PCR amplification. Therefore, hybridization among different kinds of ssDNA does not occur and nonspecific amplification and by‐products are avoided.

Article Snippet: An MJ‐Mini PCR amplification system (Bio‐Rad, CA, USA), a Bio‐Rad electrophoresis apparatus (Bio‐Rad), and a magnetic stirrer (VWR, West Chester, PA, USA) were used in the experiment.

Techniques: Amplification, Hybridization

Journal: Biotechnology and Applied Biochemistry

Article Title: Construction and optimization of an efficient amplification method of a random ssDNA library by asymmetric emulsion PCR

doi: 10.1002/bab.1467

Figure Lengend Snippet: (A) The ssDNA library obtained by asymmetric emulsion PCR. The PCR amplification cycle number ranged from 10 to 50, and the control was the ssDNA library. When the cycle number was increased from 10 to 50, sufficient amounts of products were generated and no by‐product was produced. (B) The ssDNA library obtained by conventional asymmetric PCR. The PCR amplification cycle number ranged from 10 to 50, and the control was the ssDNA library. By‐products appeared after 20 cycles. A large amount of by‐products were generated after 25 cycles, and they could not be separated from products.

Article Snippet: An MJ‐Mini PCR amplification system (Bio‐Rad, CA, USA), a Bio‐Rad electrophoresis apparatus (Bio‐Rad), and a magnetic stirrer (VWR, West Chester, PA, USA) were used in the experiment.

Techniques: Amplification, Generated, Produced